An NMR approach to tRNA tertiary structure in solution.

University of Groningen An NMR Approach to tRNA Tertiary Structure in Solution

Tertiary hydrogen bonds in the solution structure of transfer RNA.

The high resolution nuclear magnetic resonance (NMR) spectra of hydrogen-bonded protons in four tRNAs have been studied at 270 MHz. The relative intensity of the resonances between -11 ppm and -15 ppm of Escherichia coli tRNA1-Va1 indicate that there are 26 plus or minus 3 protons, while only 20 are expected from secondary structure Watson-Crick hydrogen bonds inthe cloverleaf structure. Severa...

The tRNA-like domains of E coli and A.aeolicus transfer-messenger RNA: structural and functional studies.

Transfer-messenger RNA (tmRNA, 10Sa RNA or ssrA) acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. The understanding at molecular level of this ubiquitous quality control system in eubacteria requires structural information. Here, we describe the purification and structural analysis of a func...

High - Resolution Nuclear Magnetic Resonance Determination of Transfer RNA Tertiary Base Pairs in Solution . 2 .

The number of base pairs in the solution structure of several class 111 D3VN tRNA species from E. coli has been determined by analyzing the number of low-field (15 to 11 ppm) proton resonances in their nuclear magnetic resonance spectra at 360 MHz. Contrary to previous reports indicating the absence of tertiary resonances, all the spectra exhibit the expected number of secondary base pair reson...

University of Groningen High - Resolution Nuclear Magnetic Resonance Determination of Transfer RNA Tertiary

The number of base pairs in the solution structure of several class 111 D3VN tRNA species from E. coli has been determined by analyzing the number of low-field (15 to 11 ppm) proton resonances in their nuclear magnetic resonance spectra at 360 MHz. Contrary to previous reports indicating the absence of tertiary resonances, all the spectra exhibit the expected number of secondary base pair reson...